Preparation and Characterization of Novel Perfluorooctyl Bromide Nanoparticle as Ultrasound Contrast Agent via Layer-by-Layer Self-Assembly for Folate-Receptor-Mediated Tumor Imaging

Table 3

Results of orthogonal design: the four factors were analyzed at three different levels. , , and were the average diameter of level 1, level 2, and level 3 for each factor.

Groups	A	B	C	D	The average diameter; (nm)	PDI	Zeta potential; (mV)
1	200	2.4	1.2	0.1	182.7	0.262
2	200	3.0	0	0.2	179.2	0.259
3	150	1.8	1.2	0.2	173.5	0.280
4	150	3.0	0.6	0.1	151.7	0.283
5	150	2.4	0	0.3	178.5	0.256
6	100	3.0	1.2	0.3	136.5	0.293
7	100	1.8	0	0.1	169.9	0.284
8	200	1.8	0.6	0.3	173.0	0.266
9	100	2.4	0.6	0.2	130.2	0.284
		436.6	516.4	527.6	504.3
		503.7	491.4	454.9	482.9
		534.6	467.4	492.7	488.0
		145.5	172.1	175.8	168.1
		167.9	163.8	151.6	161.0
		178.3	155.8	164.2	162.7
		32.8	16.3	23.7	7.1
		0.861	0.830	0.799	0.829
		0.819	0.802	0.833	0.823
		0.787	0.835	0.835	0.815
		0.287	0.277	0.266	0.276
		0.273	0.267	0.278	0.274
		136.5	134.9	125.4	136.9
		133.8	133.5	109.9	137.3
		47.0	47.5	58.5	45.6
		45.5	45.0	41.8	45.6
		44.6	44.5	36.6	45.8
		2.4	3.0	21.9	0.2