Preparation and Characterization of Novel Perfluorooctyl Bromide Nanoparticle as Ultrasound Contrast Agent via Layer-by-Layer Self-Assembly for Folate-Receptor-Mediated Tumor Imaging

Table 4

(a) Effect of ultrasonic intensity for PFOB nanocore template (). (b) Effect of ultrasonic time for PFOB nanocore template (). (c) Effect of ultrasonic cycles for PFOB nanocore template (). (d) Effect of ultrasonic temperature for PFOB nanocore template ().

(a)

Ultrasonic intensity (%)	The average diameter (nm)	PDI	Zeta potential (mV)
30	156.5 ± 2.4	0.279 ± 0.011	−39.3 ± 2.8
40	130.7 ± 1.8	0.248 ± 0.055	−39.4 ± 1.6
50	127.0 ± 2.3	0.305 ± 0.006	−41.8 ± 1.3

(b)

Ultrasonic time (min)	The average diameter (nm)	PDI	Zeta potential (mV)
10	131.5 ± 1.7	0.280 ± 0.006	−40.4 ± 2.4
20	105.8 ± 2.8	0.421 ± 0.024	−37.0 ± 1.1
30	110.3 ± 2.5	0.374 ± 0.040	−36.1 ± 2.5

(c)

Ultrasonic cycles (s)	The average diameter (nm)	PDI	Zeta potential (mV)
5-5	139.1 ± 2.4	0.295 ± 0.005	−37.1 ± 1.1
10-10	131.2 ± 0.4	0.281 ± 0.011	−40.1 ± 1.2
15-15	139.5 ± 1.2	0.299 ± 0.006	−36.5 ± 2.2

(d)

Temperature (°C)	The average diameter (nm)	PDI	Zeta potential (mV)
4 ± 4	166.2 ± 4.3	0.270 ± 0.007	−39.4 ± 0.8
25 ± 4	131.0 ± 1.2	0.285 ± 0.006	−40.4 ± 2.0
50 ± 4	118.5 ± 2.0	0.351 ± 0.004	−40.8 ± 2.0