Cancerous Inhibitor of PP2A Silencing Inhibits Proliferation and Promotes Apoptosis in Human Multiple Myeloma Cells

Figure 5

Effect of IGF-1 treatment on the expression level of PI3K/AKT/mTOR signaling components in RPMI-8226 and NCI-H929 cells that are knocked down by si-CIP2A. The protein expression levels of nonphosphorylated and phosphorylated PI3K p85, AKT1, and mTOR in RPMI-8226 and NCI-H929 cells that are knocked down by si-CIP2A treated with PBS or IGF-1 were evaluated by western blotting, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as a loading control.