Research Article

Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia

Table 4

Sensitivity and specificity of mecA, femA specific for S. aureus (femA-SA), femA specific for S. epidermidis (femA-SE), universal 16S rRNA, and staphylococcal 16S rRNA.

Target genesSensitivity (%)Specificity (%)

mecA98.494.5
femA-SA100.0100.0
femA-SE97.997.0
Universal 16S rRNA100.0100.0
Staphylococcal 16S rRNA100.0100.0