Modulation of Bcl-x Alternative Splicing Induces Apoptosis of Human Hepatic Stellate Cells

Figure 4

Bcl-x SSO dose-dependently induced cytotoxicity in HSCs. Human HSCs were transfected with Mock SSO or Bcl-x SSO using an Amaxa machine. Then cells were seeded into 96-well plates at the density of 5000 cells/well. After incubation of 72 hours, Alamar Blue reagent was added and incubated for another 2 hours. The plate was then read for fluorescence value using a microplate reader. Bcl-x SSO showed a dose-dependent cytotoxicity. Data are expressed as mean ± SD ().