Research Article
Cloning, Expression, and Characterization of a Novel Thermophilic Monofunctional Catalase from Geobacillus sp. CHB1
Figure 5
SDS-PAGE analyses of recombinant catalase expression and the purified enzyme. Lanes: M, protein marker; Lane 1, total bacterial protein of BL21 (DE3) cells with empty vector pEASY-E2; Lane 2, insoluble bacterial proteins of BL21 (DE3) with vector pEASY-E2-kat after induced expression; Lane 3, soluble bacterial proteins of BL21 (DE3) with vector pEASY-E2-kat after induced expression; Lane 4, SDS-PAGE analysis of purified catalase using a Ni-IDA column.