Diagnosis of Human Axillary Osmidrosis by Genotyping of the Human ABCC11 Gene: Clinical Practice and Basic Scientific Evidence

Figure 3

Effects of the SNP variant (Arg180) on the protein level and intracellular degradation of ABCC11. (a) To assess the effect of the Arg180 variant on the protein level of ABCC11, Flp-In-293 cells expressing the WT or Arg180 variant of ABCC11 were cultivated in the presence of MG132, a proteasome inhibitor, for 24 h. ABCC11 WT and Arg180 variant proteins were analyzed by immunoblotting with ABCC11-specific antibody after treatment with PNGase F, a glycosidase. The signal intensity ratio (ABCC11/GAPDH, internal control) was normalized to the control and expressed as mean ± SD. (b) Schematic illustration of the posttranslation modification of ABCC11 WT and proteasomal degradation of the Arg180 variant.

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