G2385R and I2020T Mutations Increase LRRK2 GTPase Activity

Table 1

The kinase activities of LRRK2 I2020T and G2385R mutants.

WT	I2020T	G2385R	Substrate	Detection	Reference
++	++++	++	LRRK2	Isotope	[21]
++	+++	nt	LRRK2	Isotope	[25]
++	nt	+	LRRK2	Isotope	[22]
++	nt	++	LRRK2, MBP	Isotope	[23]
++	+	1/2+	LRRK2, MBP, moesin	Isotope	[24]
++	1/2+	+	LRRK2	Antibody	[34]
++	+++	nt	LRRK2, 4E-BP1	Isotope	[35]
++	++	++	LRRKtide	Isotope	[36]
++	+++	+	LRRK2, Rab5B	Isotope	This study

+: assigned based on the graphic values of each mutant from the indicated studies.
nt: not tested.
All previous experiments cited here have used immunoprecipitates of cell lysate that was transiently transfected with full length LRRK2 except [24] which has used GST-LRRK2 [1326–2527] for transfection. The kinase activities have been detected by either [γ-] ATP or phospho-LRRK2 antibodies such as pS910 or pS935.
The WT activity of each study was arbitrarily assigned as ++ and compared within each study, not between studies.