The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived EPCs and BMSCs in Osteogenesis and Angiogenesis

Table 1

Primer and PCR conditions used for qRT-PCR.

Genes	Primer sequence	Lengths of products
VEGF	Forward: 5′ GAAGAAGGAGACAATAAACCC 3′; Reversal: 5′ ACCAGAGGCACGCAGGAA 3′	152 bp
Osteopontin	Forward: 5′ GCTCAGCACCTGAATGTACC 3′; Reversal: 5′ CTTCGGCTCGATGGCTAGC 3′	247 bp
Osteonectin	Forward: 5′ CTCCAGCTGGACTACATCG 3′; Reversal: 5′ CTCCATGGGGATGAGTGGT 3′	369 bp
Collagen Type I	Forward: 5′ TCAACGGTGCTCCTGGTGAAG 3′; Reversal: 5′ GGACCTTGGCTACCCTGAGAA 3′	514 bp
β-actin	Forward: 5′ GCTCGTCGTCGACAACGGCTC 3′; Reversal: 5′ CAAACATGATCTGGGTCATCTTCTC 3′	353 bp