Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots

Figure 5

Phylogenetic analysis of L. sericata caspases. The phylogenetic analysis of eight identified caspases in the transcriptome of L. sericata. For the analysis the sequences were compared with Bombyx mori (BOMMO), UniProtKB [E0D2V3; E9JEH0; E0D2V3; E9JEH0; Q2HZ05; Q8I9V7], Tribolium castaneum (TRICA), UniProtKB [D6WD84; D6WD86; D6WFK4; D6WIV9; D6WJH5; D6X3B3], Musca domestica (MUSDO), UniProtKB [T1P9A0; T1PAQ7; T1PFC3; T1PK82; T1PN44; B5AK94], Bactrocera cucurbitae (BACCU), UniProtKB [A0A0A1WTD1; A0A0A1X1L7; A0A0A1X8B8; A0A0A1XL94; A0A0A1XM78; A0A0A1XQW9; A0A0A1XRT4], D. melanogaster (Strica, UniProtKB [Q7KHK9]; Dronc, UniProtKB [Q9XYF4]; Dredd, UniProtKB [Q8IRY7]; Damm, UniProtKB [O44252]; Drice, UniProtKB [O01382]; Dcp-1, UniProtKB [O02002]; Decay, UniProtKB [Q9VET9]), and Anopheles gambiae (agL1, agL2, ags1–ags14), UniProtKB [Q5TMK1; Q7Q2Q0; Q5TMS0; Q7PZJ9; Q7PZK0; Q7PV92; Q7Q802; Q7Q803; Q7Q4X6; Q7PWK2; Q7QGM9; Q7PSJ2; Q7Q801; Q7QGN0; Q7QGM8; Q7QKT2]. For the alignment MAFFT [199] was used.