Antiobesity and Antidiabetes Effects of a Cudrania tricuspidata Hydrophilic Extract Presenting PTP1B Inhibitory Potential

Figure 1

Effect of CTe on PTP1B activity and lipid droplets. (a) The substrate (IR; insulin receptor) was subjected to autophosphorylation for 1 h, followed by spotting on a chip slide. Reaction mixture was incubated for 24 h in the presence of PTP1B (100 mg/mL) with sodium orthovanadate (Na₃VO₄; 800 μM), CTe (50, 100, or 200 μg/mL), or 10% PEG alone. The fluorescent signal corresponding to the phosphorylated IR incubated with an anti-phospho-IR, followed by incubation with an anti-Cy5 antibody, was measured using a microarray scanner system. (b) CTe inhibition of fat droplet formation in 3T3-L1 cells. 3T3-L1 cells were stained with Oil Red O dye and examined using a light microscope. (c) Quantification of lipid accumulation in differentiated 3T3-L1 cells by measuring the absorbance of the cell extract at 520 nm. Statistical significance was determined by Student’s -test. and versus MDI treated cells.

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