Discovery of Azurin-Like Anticancer Bacteriocins from Human Gut Microbiome through Homology Modeling and Molecular Docking against the Tumor Suppressor p53

Table 1

Hydrophobic percentages (HPP) of 14 most putative azurin-like bacteriocins.

Number	Sequence ID	BAGEL ID	Bacteria species/strain	Amino acid sequence	HPP (%)
	Azurin		Pseudomonas aeruginosa PAO1	MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIAHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK	49
	p28-azurin			LSTAADMQGVVTDGMASGLDKDYLKPDD	46
1	p2seq05	AOI 1 orf003	Anaerotruncus colihominis DSM 17241	MEKPAVESIVKTAGNGTKRWYNRMKKQTAAITAVVCVFLFATHTQALEVPARVTRTVTTTQKIPFATSYIDLPGIYRGYEEPVRSGTPGEQKIEAQVIYEGDRAVKVVSIKAEQTAQPVNAVVKRGTKVLYSETADGSAWKTSFARPLKGGWLSADFYDYPHHNGIDLAAPYGTPVYAAAEGVVEQAGWYGEYGICVILRHADGSRTLYGHNSSVSVSVGQTVKQGEKIANVGSTGNSTGNHLHFEIRVDGRMIDPLVYLDQ	46
2	p3seq17	AOI 1 smallORF 28	Bacteroides vulgatus PC510	MFHHSCLVMNIHIWECMSTTLAPQQQRVTLTVITGIVCFLSHTYQTTIRVLALSGTDSFTNNGTTGISA	49
3	p3seq16	AOI 1 smallORF 29		MSTTLAPQQQRVTLTVITGIVCFLSHTYQTTIRVLALSGTDSFTNNGTTGISA	45
4	p3seq24	AOI 1 orf007	Clostridium hathewayi DSM 13479	LEELIVNLVQSQGIWAVLFVFLLLYTIKKNDKLDELQEARERKYQELLTQLTVKLSIVNTVNEKLDTIQAVLKEKSD	45
5	p2seq14	AOI 1 smallORF 20	Clostridium nexile DSM 1787	MGLLEILPIIPYLLVRACDCTASDRTNTTYSCRRT	46
6	p2seq08	AOI 1 orf006		VERTFSLSKRCYGMSCITTKLEETQLTSIALSVFVTNLFRIQRRILCALLHLFRFWYDRNRYKSWKLQIAA	48
7	p2seq33	AOI 1 orf021		MLKKKVKKYLLISGISFAIGTLGIIFVSVLIEEVVRAIAGEEANKQITQSDLEGLPAWITVEMVQAAIDMMNETGYPASVVLGQMILEAGADGSELANPPYYNCLGQKAHCYKENGTVVMRTEEAWGTVTAEFSTFANYVDCMLAWGNKFTRQPYVDNVTACKRDPVTGHYDADSFITALWKSGYATDPAYVSKVIAVMKSRNLYRFNYMTSADLENGLGEIGTGMFTHPCPGMTYQSSYFGEIREFETGGHKGNDYAAPAGTPTLAAADGTVTVAGWSDSAGNWVVIDHGNGLTTKYMHHSRLLVKTGDTVKKGQQIGEVGSTGQSTGNHLHFQVEENGVPVNPDKYLKGEGNERE	48
8	p2seq32	AOI 1 orf024		MLRKKVKKYLLISGISFAIGILGIIFVSTLIEEVVRAIAGEEANKQIVQSDLDGLPAWITVEMVQAAIDMMNETGYPASVVLGQMILEAGADGSELANPPYYNCLGQKAHCYKESGTVVMRTEEAWGTVTAEFSTFANYVDCMLAWGNKFTRQPYVDNVIACKRDPVTGHYDADSFITALWKSGYATDPAYVSKVIAVMKSRNLYRFNYMTSADLENGLGEIGNGMFTHPCPGMTYQSSYFGEIREFETGGHKGNDYAAPAGTPTLAAADGIVTIAGWSDSAGNWVVIDHGNGLTTKYMHHSKLLVKTGDTVKKGQQIGEVGSTGQSTGNHLHFQVEENGVPVNPDKYLKGDGE	49
9	p2seq18	AOI 1 orf061		VFTSFSLSSIATLPRLKEIILTSCFKHHFKEYTTLSISASPFLSNICILIISLSAKYFFIIAAHIPPCIVVQLSRQKSKLFIMN	51
10	p2seq20	AOI 2 smallORF 1		MVAFVVISPITRTRPVLIATSHATRLSGSCLIHSSNTASAI	51
11	p1seq09	AOI 1 smallORF 1	Dorea longicatena DSM 13814	MKRAATCHSHQHLTGLAERYAARHTSGSLQFLFFF	49
12	p1seq16	AOI 1 orf012	Eubacterium ventriosum ATCC 27560	NKKSHKSAFVCIAVFMVTMIMILTFYQLAVEIYAYGFIAKNNDYRVKESTAKVLTLKENHKAINIENINVNSKTKVINKKNINVNNFVLKKPVKGGITTSGFGDTISRTASHNGHDWAVNTGTKVRAAAEGVVELAYFSESYGYNILINHNNGFKTRYAHLSEVKVSKGEKVEQSQVIALSGSTGFSTGPHLHFEVVKDGKRVNPIEYVSNR	44
13	p3seq02	AOI 1 smallORF 15	Ruminococcus sp. 5.1.39.B FAA	MQVYSLFIFSASVYCKIITGKEDIRCSGTYN	48
14	p3seq04	AOI 1 smallORF 20		MLNAAGCFRISFPTSDSDSLYSSFTVKTVAE	45