Research Article

A Quantitative Real-Time RT-PCR Assay for the Detection of Venezuelan equine encephalitis virus Utilizing a Universal Alphavirus Control RNA

Figure 3

Comparison of the consensus sequences of different VEEV subtypes. (a) Sequences of synthetic RNA constructs (sVEEV) encompass the target region of the VEEV specific qRT-PCR. Nucleotides with mismatch to the reference sequence are indicated. (b) Standard curves of serial diluted sVEEV were obtained by Ct values plotted against the log of diluted template.
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