The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells

Figure 2

Stimulating effect of MB salt on the AMPK pathway. After serum starvation, cells were treated with different concentrations of MB salt for 24 h (a). Alternatively, cells were pretreated with CC for 30 min; then MB salt was added and incubated for 24 h (b). In the comparison experiment, an equal concentration of MB salt/M/B was used to treat the cells for 24 h (c). DMSO (0.1%) was used as control. After treatment, cell total proteins were extracted; the levels of p-AMPKα (Thr172), AMPKα, p-ACC (Ser79), ACC, and ACTB were determined by western blot. Representative blots are presented. The protein levels of p-AMPKα (Thr172) and p-ACC (Ser79) were normalized to those of AMPKα and ACC, respectively, and plotted as fold of DMSO treated cells. Values are mean ± SD of 3 separate experiments; and versus that of DMSO; versus that of MB salt alone in (b); versus those of M alone or B alone in (c).

(a)

(b)

(c)