Thrombin Maybe Plays an Important Role in MK Differentiation into Platelets

Figure 3

Thrombin inhibits cell proliferation and induces G0/G1 arrest and apoptosis in Meg-01 cells. (a) Meg-01 cell proliferation was assayed by CCK-8. Meg-01 cells were treated with 80 ng/mL TPO or 2 U/mL thrombin for 24 h. (b) After treatment with 80 ng/mL TPO or 2 U/mL thrombin for 24 h, the cell cycle distribution of Meg-01 cells was determined by flow cytometry. (c) The percentage of apoptotic cells was detected via Annexin V/PI staining after cells were treated with thrombin or TPO for 24 h. The data show mean ± SD from three independent experiments. < 0.05 and < 0.01, compared with the control.

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