Effect of Water-Glass Coating on HA and HA-TCP Samples for MSCs Adhesion, Proliferation, and Differentiation

Table 2

Primers and PCR conditions.

	Sense (5′-3′)	Antisense (5′-3′)	Annealing/cycle
Fibronectin (FN)	AGT TCA GGG TTC CTG GAA	TGC CAC TGT TCT CCT ACG TG	56°C/25
E-cadherin (E-cad)	CAA GGA CAG CCT TCT TTT CG	TGG ACT TCA GCG TCA CTT TG	57°C/38
Osteocalcin (OC)	CCC TCA CAC TCC TCG CCC TAT	TCA GCC AAC TCG TCA CAG TCC	59°C/30
Osteopontin (OPN)	CCA AGT AAG TCC AAC GAA AG	GGT GAT GTC CTC GTC TGT A	57°C/30
α-smooth muscle actin (α-SMA)	ACT GTG TTA TGT AGC TCT GGA C	ACA ATG GAA GGC CCG GCT TC	57°C/25
GAPDH	AGG TCG GAG TCA ACG GAT TTG	GTG ATG GCA TGG ACT GTG GT	58°C/25