Review Article

Biotechnological Processes in Microbial Amylase Production

Figure 7

Amylase production on agar plate. In this solid-based method, the starch-containing agar plate is pinpoint-inoculated with the microorganism at the center of the Petri plate. After an appropriate incubation period, flooding the plate with iodine solution reveals a dark bluish color on the substrate region. The clear region around the inoculum indicates the zone of hydrolysis. (a) Amylolytic activity by Aspergillus versicolor; (b) amylolytic activity by Penicillium sp.