Role of Phosphorylated HDAC4 in Stroke-Induced Angiogenesis

Figure 4

Phosphorylated HDAC4 regulates HIF-VEGF downstream genes expression. ((a) and (b)) Expression change of HIF1α, VEGFa, and P-HDAC4 in the ischemic penumbra cortex and contralateral cortex at the indicated time points. Quantitative analysis is shown in (b). ((c) and (d)) Expression change of HIF1α, VEGFa, and P-HDAC4 in RBMEC cells cultured in the model of hypoxia. Quantitative analysis is shown in (d). (e) Nur77 and Rcan2 mRNA expression was determined after GÖ6976 or vehicle injection (/group). Data are presented as mean ± SD. NS, nonstatistically significant. versus L. (f) RBMEC cells treated with GÖ6976 or vehicle control were cultured for 24 hours. The expression of Nur77 and Rcan2 was quantified by qRT-PCR at the indicated time points. Data are presented as mean ± SD. versus N; versus H.

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