Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease

<div>Optimization of the amount of antiserum and lysate to be used in the indirect ELISA. The amount of control lysate coated and antiserum dilution used were tested at 10-fold ranging from 10 to 0.01 <i>μ</i>g concentration and from 1 : 100 to 1 : 100,000 dilution, respectively, in a checkerboard titration.</div>

BioMed Research International

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Figure 1

Figure 1: Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease 