Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease

Figure 3

Leaf sap titration. Both membranes were spotted with the same samples and in the same order. The first three samples from the left were leaf sap from infected TN1 diluted twofold (1 : 2.5, 1 : 5, and 1 : 10) with 1x PBS pH 7.4. The last three samples were leaf sap from healthy TN1 subjected to the same treatment. Membrane in (a) was probed with positive antiserum while membrane in (b) was probed with negative antiserum. The last row was spotted with control lysates, positive and negative diluted 1 : 4 with 1x PBS.

(a)

(b)