CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells

Figure 2

High glucose induces the activation of NF-κB signaling and release of MCP-1, IL-6, and IL-8. (a) Protein expressions of IκBα, p-IκBα, NF-κBp65, and p-NF-κBp65 in lysates of mouse GMCs treated with 30 mmol/L glucose for 6, 12, 24, 48, and 72 h were detected by Western blot. (b) Protein expressions of IκBα, p-IκBα, NF-κBp65, and p-NF-κBp65 in lysates of mouse GMCs treated with an indicated concentration of high glucose for 24 h as detected by Western blot. (c) MG132 partially reversed high glucose-induced IκBα ubiquitination degradation and NF-κB activation. (d) Release of the inflammatory cytokines MCP-1, IL-6, and IL-8 in the cell culture supernatant was quantified by ELISA. Data were normalized with respect to β-actin and are expressed as mean ± SD. The gray graphs show the relative statistical values in each group and confirmed these trends. compared with NC group; compared with 30 mmol/L glucose (HG3) group.

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