CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells

Figure 3

siRNA-mediated knockdown CYLD facilitated the activation of NF-κB induced by high glucose. (a) RT-PCR and Western blot detected the mRNA and protein expression of CYLD in mouse GMCs treated with 30 mmol/L glucose and (or) CYLD siRNA for 24 h. (b) Western blot detection of the expression of IκBα, p-IκBα, NF-κB, and p-NF-κB in mouse GMCs treated with 30 mmol/L glucose and (or) CYLD siRNA for 24 h. Data were normalized with respect to β-actin and are expressed as mean ± SD. The gray graphs show the relative statistical values in each group and confirmed these trends. compared with the NC group; compared with the 30 mmol/L glucose stimulation group.

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