CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells

Figure 4

Overexpression of CYLD reverses high glucose-induced activation of the NF-κB signaling pathway. (a) RT-PCR and Western blot detection of the mRNA and protein expression of CYLD in mouse GMCs treated with 30 mmol/L glucose and (or) overexpression of CYLD for 24 h. (b) Western blot detection of the expression of IκBα, p-IκBα, NF-κBp65, and p-NF-κBp65 in mouse GMCs treated with 30 mmol/L glucose and (or) overexpression CYLD for 24 h. (c) ELISA detection of the release of the inflammatory cytokines MCP-1, IL-6, and IL-8 from mouse GMCs treated with 30 mmol/L glucose and (or) overexpression of CYLD for 24 h. The gray graphs show the relative statistical values in each group and confirmed these trends. compared with the NC + blank load transfection (NC + LV-CON235) group; compared with 30 mmol/L glucose + LV-CON235 group.

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