Identification of Streptococcus pneumoniae: Development of a Standardized Protocol for Optochin Susceptibility Testing Using Total Lab Automation

Figure 1

DOIZs (diameters of inhibition zones) by agar (figures) and imaging time point (bars); COS_BD: Columbia agar 5% sheep blood (Becton Dickinson), COS_bmx: Columbia agar 5% sheep blood (bioMerieux), MHB_bmx: Müller-Hinton agar with 5% sheep blood (bioMerieux), MHF_bmx: Müller-Hinton agar fastidious with 5% horse blood (bioMerieux), MHF_Ox: Müller-Hinton agar fastidious with 5% horse blood (Oxoid), TSA_bmx: Tryptic Soy agar with 5% sheep blood (bioMerieux), and TSA_Ox: Tryptic Soy agar with 5% sheep blood; green line: 14 mm cut-off.