Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells

Figure 1

Strategy to eliminate a copy of chromosome 21 in HeLa cells. (a) Image of HeLa cell nuclei indicating three signals (green) by FISH using a chromosome 21 specific probe. DNA was stained with DAPI. (b) Scheme of recombination at inverted loxP sites by Cre recombinase. DNA cassette was integrated into chromosome 21 by homology-directed repair induced CRISPR/Cas9 nickase system. Cre-mediated recombination at the loxP sites between sister chromatids produced a dicentric and an acentric chromosome. Neo: neomycin resistance gene.

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