Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells

Figure 2

Recombination analysis between loxP sites by Cre recombinase. PCR primer-binding sites were shown on acentric chromosome and normal chromosome 21. PCR amplification was performed using genomic DNA isolated from the cells with and without integration of the DNA cassette before (Cre−) or 3 days after transfection of pCMV-Cre (Cre+).