First Characterization of the Neospora caninum Dense Granule Protein GRA9

Figure 5

(a) shows a low magnification view of LR-White embedded N. caninum tachyzoites forming an intracellular PV in HFF. The boxed areas in (a) are shown at higher magnification in (b), (c), and (d). dg = dense granules, rop = rhoptries, mic = micronemes, and nuc = nucleus. (e) and (f) show another PV, where the intravacuolar membranous network is stained with anti-NcGRA9 antibodies (arrows). (e) shows the boxed area in (f) at higher magnification. Bars in (a) = 1 μm, (b) = 0.14 μm, (c) and (d) = 0.2 μm, (e) = 1.8 μm, and (f) = 1.6 μm.