Increased Circulating Th17 but Decreased CD4⁺Foxp3⁺ Treg and CD19⁺CD1d^hiCD5⁺ Breg Subsets in New-Onset Graves’ Disease

Figure 1

The frequencies of circulating Th17 cells, CD4⁺Foxp3⁺ Tregs, and CD19⁺CD1CD5⁺ Bregs and the ratio of Th17/Treg in patients and healthy controls. PBMCs from participants’ peripheral blood unstimulated or stimulated with PMA plus ionomycin were stained with a panel of fluorochrome-conjugated monoclonal antibodies against CD4, IL-17, and Foxp3 using Human Th17/Treg phenotype Kit or against CD19, CD5, and CD1d. In the three-color fluorescence cytometric analysis, cells were gated first on lymphocytes, then the CD4⁺ T cells were gated out for further IL-17⁺ and Foxp3⁺ T cell subsets analysis (a), and the CD19⁺ B cells were gated out for further CD1CD5⁺ B cell subset analysis (c). The quantitative analysis of circulating CD4⁺IL-17⁺, CD4⁺Foxp3⁺ T cells and CD19⁺CD1CD5⁺ B cells as well as the ratio of Th17/Treg were shown in (b) and (d). The quantitative analysis of individual values were shown as mean ± SD. A value < 0.05 was considered statistical difference.

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