An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos

Figure 1

Sox9, Sox5, and Sox6 gene targeting and their E13.5 transgenic embryos generated for FACS and expression Profiling. (a) Wildtype Sox9 allele (Sox9⁺) is indicated with exons depicted as black boxes. Translation start site (ATG) as indicated. (b) Sox9 with the F2A-EGFP-FRTNeo inserted in exon 3 and after FLPe recombination . E13.5 embryo shown on the right. (c) Sox9-null allele with the EGFP-loxPNeo inserted after the ATG and after CRE recombination . Sox9-null allele with FRTNeo inserted in exon 1 and after FLPe recombination . E13.5 chimeric embryo shown on the right. (d) Exon containing the ATG of wildtype Sox5 (Sox5⁺) shown. EGFP-loxPNeo inserted after the ATG () and after CRE recombination . E13.5 embryo shown on the right. (e) Exon containing the ATG of wildtype Sox6 (Sox6⁺) shown. EGFP-loxPNeo inserted after the ATG and after CRE recombination . E13.5 embryo shown with a wildtype embryo (WT) under same lighting condition. EGFP, green boxes; F2A, blue ovals; FRT sites, red triangles; loxP sites, yellow triangles (see also Figure S1 and Table S1).