Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor Liver Transplantation by Laser Capture Microdissection and Pyrosequencing Analysis

Table 2

The results of the pyrosequencing investigation of the interleukin 28B single nucleotide polymorphism rs8099917 genotypes on the isolated hepatocytes from the liver graft biopsy and the serum from the recipients on the postoperative day 0 and the relationship of the donor’s genotype.

Donor (serum)	Liver graft (cell)			POD0 (serum)
Genotype	G freq. (%)	T freq. (%)	Genotype	G freq. (%)	T freq. (%)	Genotype
GG	14.45	85.55	GT	3.36	96.64	TT
TT	5.98	94.02	TT	2.34	97.66	TT
TT	2.7	97.3	TT	2.07	97.93	TT
GT	16.54	83.46	GT	3.15	96.85	TT
GT	66.43	33.57	GT	59.02	40.98	GT

Genotype = genomic DNA that is identified by the Applied Biosystems International, 7500 real-time polymerase chain reaction; freq. = frequency (pyrosequencing method, PyroMark, Qiagen).