Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial–Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α

Figure 4

HIF-1α knockdown partially reversed hypoxia-induced docetaxel resistance. (a–c) Cell viability assay quantification of prostate cancer cells treated with docetaxel with or without propofol under normoxic or hypoxic conditions following HIF-1α knockdown. (d–f) EdU assay detection of the proliferation rate of prostate cancer cells following HIF-1α knockdown and treatment with docetaxel, docetaxel plus propofol in hypoxia, or docetaxel alone in normoxia. (g) Western blot assessment of HIF-1α knockdown efficiency in hypoxia condition; histogram represents the average grey value of the HIF-1α protein. versus control. DTX, docetaxel; ns, not significant.