Identification of Key Candidate Proteins and Pathways Associated with Temozolomide Resistance in Glioblastoma Based on Subcellular Proteomics and Bioinformatical Analysis

Figure 1

Cytoplasmic proteome research of U87 GBM cells treated with TMZ for 1 week. (a) Morphology and viability change of U87 cells after temozolomide treatment for 1 week; (b) outline of the experimental workflow; (c) verification of the purity of nucleus and cytoplasma extractions; (d) volcano figure of all identified proteins. Red plots mean upregulated proteins in cytoplasma after TMZ treatment, and purple plots mean downregulated proteins. and value < 0.05 were considered as the cutoff values for DEPs screening; (e) Heat map of the significant DEPs.

(a)

(b)

(c)

(d)

(e)