De Novo Endotoxin-Induced Production of Antibodies against the Bile Salt Export Pump Associated with Bacterial Infection following Major Hepatectomy

Figure 5

Indirect immunoblot assay to test the presence of antibodies against bile salt export pump (BSEP) in the sera after hepatectomy (HT). (a) Membrane protein lysate from normal liver tissue was subjected to western blot analysis with control anti-BSEP antibodies (BSEP Ab) or sera obtained from experimental mice. Blots with sera as primary antibodies and with peroxidase-labeled anti-mouse IgM as secondary antibodies showed that the presence of BSEP antibodies was detected at postoperative day 6 in mice that had undergone 70% HT and LPS treatment. The experiments were done by triple lanes with the same protein sample from normal liver tissue. Each stripe represented intensity of BSEP antibody within sera from experimental mouse as shown by legend in the left-hand side. (b) Quantification intensity of BSEP antibody expression within sera. Mice that had not undergone HT and mice that had undergone HT without LPS treatment had no detectable BSEP antibodies in sera at the same time point. Arrow indicates BSEP (146 kDa).

(a)

(b)