The Effect of Breed, Gender, and Acid Stimulation in Dog Saliva Proteome

Table 5

Mass spectrometry identification of proteins present in spots from saliva 2-DE profiles differing between stimulation conditions and/or among breeds.

Spots	Protein	Entry; reference	Estim/; theoret; MW (kDa)	Estim/; theor pI	Score	% Seq. Cov.	Matched Peptides; MS (MS/MS)
0	Chain A, Crystal Structure Analysis Of Canine Serum Albumin	pdb|5GHK|A (NCBI)	78.1/65.7	4.9/5.3	263	42	18 (2)
5	Keratin, type I cytoskeletal 10	Q6EIZ0 (Uniprot)	18.5/57.7	7.8/5.1	207	30	10 (5)
8	double-headed protease inhibitor, submandibular gland	XP_022264993.1 (NCBI)	17.9/15.7	6.0/8.6	428	58	4 (8)
12	Immunoglobulin J chain	XP_532398.2 (NCBI)	30.1/18.3	4.4/4.7	125	40	3 (2)
16	Immunoglobulin lambda-1 light chain isoform X34	XP_005636600.1 (NCBI)	30.0/24.8	6.0/6.4	326	33	6 (4)
18	Immunoglobulin lambda-1 light chain isoform X25	XP_022266294.1 (NCBI)	31.0/24.9	5.5/5.1	198	35	8 (4)
37	IgGFc-binding protein	XP_022261796.1 (NCBI)	59.9/318.0	4.9/5.2	267	4	7 (4)
45	Uncharacterized protein	J9P732 (Uniprot)	25.0/21.4	5.8/6.0	192	28	4 (5)
81	Uncharacterized protein	F1PW98 (Uniprot)	19.1/55.0	8.0/5.7	111	29	15 (2)

score is log, where is the probability that the observed match is a random event. Protein scores greater than 62 are significant  .