Involvement of P2X₇ Receptor in Proliferation and Migration of Human Glioma Cells

Figure 3

BzATP enhances migration and proliferation of U87 and U251 glioma cells. (a) Increased migration of the U87 cells with the treatment of BzATP and blocked by BBG (antagonist of P2X₇R), P2X₇R-siRNA, and PD98059 (ERK inhibitor) for 24 h. (b) Bar charts summarizing the migration of U87 cells under different conditions. (c) Increased migration of the U251 cells with the treatment of BzATP and blocked by BBG, P2X₇R-siRNA and PD98059 for 24 h. (d) Bar charts summarizing the migration of U251 cells under different conditions. (e) A strong expression of P2X7R was detected in the scrambled siRNA treated groups and its expression was almost absent in the P2X₇R-siRNA treated groups. (f) The expression of p-ERK was suppressed by PD98059. (g) The proliferation mediated by BzATP of U87 glioma cell was blocked by P2X₇R-siRNA. (h) The proliferation mediated by BzATP of U251 glioma cell was blocked by P2X₇R-siRNA. compared with the control; compared with the BzATP group.

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