Involvement of P2X₇ Receptor in Proliferation and Migration of Human Glioma Cells

Figure 5

protein expression was induced by BzATP in human glioma cells. (a) Immunofluorescence labeling showing P2X₇R protein expression in control (Ctr) and BzATP (100 μM)-treated U87 cells for different time (6–48 h). Scale bar = 20 μm. (b) Representative immunoblots showing P2X₇R protein expression in Ctr and BzATP (100 μM)-treated U87 cells for different time (6–48 h). (c) Bar charts summarizing the average densitometric quantification of immunoreactive bands of P2X₇R proteins of U87 cells under different conditions, for each group. versus Ctr. (d) Immunofluorescence labeling showing the changes in P2X₇R protein expression in Ctr and BzATP (100 μM)-treated U251 cells for 24 h. Scale bar, 20 μm for all images. (e) Representative immunoblots showing the changes in P2X₇R protein expression in Ctr and BzATP (100 μM)-treated U251 cells for 24 h. (f) Bar charts summarizing the average densitometric quantification of immunoreactive bands of P2X₇R proteins of U 251 cells in Ctr and BzATP (100 μM)-treated groups, for each group. versus Ctr.

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