Stromal Cell-Derived Factor-1α Alleviates Calcium-Sensing Receptor Activation-Mediated Ischemia/Reperfusion Injury by Inhibiting Caspase-3/Caspase-9-Induced Cell Apoptosis in Rat Free Flaps

Figure 3

The protein expression levels of p-CaSR, CXCR4, FAS, Cyt-c, caspase-9, Bax, Bcl-2, and cleaved caspase-3 were assessed by western blot analysis after 2 h or 7 d of reperfusion. The expression levels of p-CaSR, FAS, Cyt-c, caspase-9, and Bax were highest in the CaCl₂ group, while the expression levels of these proteins were significantly lower in the NPS2143 + CaCl₂ and SDF-1α + CaCl₂ groups. The expression levels of p-CaSR showed a significant difference at hour 2 but not on day 7 after I/R of free flaps. The expression level of CXCR4 was the strongest while that of caspase-9 was the weakest in the SDF-1α + CaCl₂ group over time. versus CaCl₂ group, versus CaCl₂ group, versus SDF-1α + CaCl₂ group, versus SDF-1α + CaCl₂ group.