Bromodomain and Extraterminal (BET) Protein Inhibition Restores Redox Balance and Inhibits Myofibroblast Activation
Figure 1
Effect of SOD2 siRNA knock-down on αSMA expression and cell proliferation. (a) Levels of SOD2 mRNA in non-ILD control (C1-C6), SSc-ILD (S1-S4), and IPF (U1-U3) lung fibroblasts under basal serum-free conditions as determined by Affymetrix microarray analysis (black bars) were confirmed by RT-qPCR (grey bars). (b-d) Non-ILD control lung fibroblasts were either mock-transfected (siRNA –ve) or transfected with nontargeting siRNA (control siRNA), or SOD2-targeting siRNA for 72 h following which SOD2 mRNA and protein (inset) (b) and (c) ACTA2 mRNA expression levels were measured. (d) Proliferation induced by incubation with 3% FBS for 24 h was measured by BrdU incorporation. Data are shown as the mean of three independent experiments performed in two control cell lines ((b) and (c)) or in one control cell line (d), respectively.