Bromodomain and Extraterminal (BET) Protein Inhibition Restores Redox Balance and Inhibits Myofibroblast Activation

Figure 4

Effect of JQ1 on TGF-β-induced NOX4, SOD2 and ACTA2 mRNA expression, and intracellular ROS. Non-ILD control pulmonary fibroblasts were serum starved and pretreated with 500 nM JQ1(+) or JQ1(-) for 2 h before stimulation with TGF-β1 (1ng/ml) for further 24 h. (a) NOX4, SOD2, and ACTA2 mRNA was determined by RT-qPCR. (b) Intracellular ROS levels were determined by 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate staining. Data are expressed as fold change compared to the DMSO control and bars represent mean ± SD of independent experiments performed in three (a) and four (b) cell lines. p<0.05, p<0.01, and p<0.001.

(a)

(b)