Glypican-3 Enhances Reprogramming of Glucose Metabolism in Liver Cancer Cells

Figure 4

HIF-1α is essential for the effect of GPC3 in glucose metabolism reprogramming. (a and b) qRT-PCR and western blot analyses for expression levels of p53, MYC, and HIF-1α in HLE and HLF cells with treatment as indicated. (c) Spearman correlation analysis between the expression levels of GPC3 and HIF-1α in 50 LC tissues based on the results from IHC staining. (d) Western blot analysis for expression levels of HIF-1α, Glut1, HK2, LDH-A, and PGC-1α in HLE cells with treatment as indicated. (e) Glucose uptake, lactate production, and pH value in the culture medium were measured in HLE cells with treatment as indicated. (f) Oxygen consumption rate (OCR) was measured in HLE cells with treatment as indicated. (g) Relative enzyme activities of respiratory complexes I–V were measured in HLE cells with treatment as indicated. Data shown are the mean ± SEM from three independent experiments. ; .

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