Effect of Hypoxia on Gene Expression in Cell Populations Involved in Wound Healing

Figure 1

Hypoxia effect on cell viability. HaCaT, HDF, HMEC-1 and differentiated THP-1 were incubated for 24 h in normoxia (black bars) or hypoxia (1% O2, gray bars). The percentage of cell viability was measured using the MTT assay (panel (a)), whereas the percentage of cytotoxicity was calculated measuring the release of LDH (panel (b)). Data are shown as mean ± standard deviation of three independent experiments. Two-tailed Student’s t-test was performed to compare viability and cytotoxicity in normoxia vs hypoxia.

(a)

(b)