Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured In Vitro with Silver Doped Bioactive Glasses Microparticles

Figure 4

Cytokine release by PBMC cultures incubated with BG or BGAg. Titration of TNF-α (a), IL-1β (b), IL-6 (c), IL-4 (d), and IL-10 (e) released at 24-hour culture supernatant of PBMC’s cultured with or without 5 μg/ml PHA stimulation. There were no significant differences on cytokine’s production between BG and BGAg stimulus and medium alone treatment. Bioactive glasses were unable to reduce cytokine levels after PHA coincubation. Correspond to the statistical difference (p<0,05). Data were presented as the median ± 95% CI of triplicates of each volunteer (n=3).

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