Antitumor Effect of Albendazole on Cutaneous Squamous Cell Carcinoma (SCC) Cells

Figure 5

Albendazole-induced apoptosis is via the ER stress. (a) SCC12 and SCC13 cells were treated with 1 μM of albendazole for the indicated time points. The ER stress markers were detected by Western blot. Albendazole induced the ER stress in a time-dependent manner. (b) SCC12 and SCC13 cells were pretreated with ER stress inhibitor 4-PBA at the indicated concentrations for 1 h and then treated with 1 μM of albendazole for 24 h. Cell viability was measured by MTT assay. Inhibition of ER stress by 4-PBA attenuated albendazole-induced cell death. Data are expressed as percentage of control. The mean values ± SD are averages of triplicate measurements. P<0.01. (c) Cells were treated as in (b); then ER stress markers were detected by Western blot.

(a)

(b)

(c)