Antitumor Effect of Albendazole on Cutaneous Squamous Cell Carcinoma (SCC) Cells
Figure 7
Effect of albendazole on cancer stemness. (a) SCC12 and SCC13 cells were treated with albendazole at the indicated concentrations for 24 h. Cells were then detached from culture dishes and then reseeded at a low concentration (3,000 cells per dish). Cells were cultured for 2 weeks and stained with crystal violet. Colony numbers were counted and plotted in right graph. Albendazole decreased the colony-forming ability of SCC cells. Data are expressed as percentage of control. The mean values ± SD are averages of triplicate measurements. P<0.01. (b) SCC12 and SCC13 cells were transduced with TOPflash reporter adenovirus and then treated with albendazole at the indicated concentrations for 24 h. The cell lysates were assayed for luciferase activities. Albendazole decreased the TOPflash activity at sublethal doses. Data are expressed as percentage of control. The mean values ± SD are averages of triplicate measurements. P<0.01. (c) SCC12 and SCC13 cells were treated with albendazole at the indicated concentrations for 24 h. The protein levels of β-catenin were detected by Western blot. Albendazole decreased the protein level of β-catenin. α-Tubulin was used for internal control.