Eya2 Is Overexpressed in Human Prostate Cancer and Regulates Docetaxel Sensitivity and Mitochondrial Membrane Potential through AKT/Bcl-2 Signaling

Figure 5

Eya2 upregulates Bcl-2 via the AKT signaling pathway. (a) We identified positive associations between Eya2 mRNA and Bcl-2/MMP7 mRNA in 497 cases of prostate cancer (Pearson's correlation analysis, RNA-seq data was obtained from the TCGA database). (b) Protein levels of Bcl-2, p-AKT, and AKT in LNCaP cells treated with Eya2 and the AKT inhibitor, perifosine. Realtime PCR analysis of Bcl-2 mRNA in LNCaP cells treated with Eya2 plasmid and the AKT inhibitor, perifosine. Eya2 overexpression failed to upregulate levels of Bcl-2 mRNA and protein in cells treated with the AKT inhibitor.

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