Antiplasmodial Activity Assay of 3-Chloro-4-(4-chlorophenoxy) Aniline Combinations with Artesunate or Chloroquine In Vitro and in a Mouse Model

Table 3

In vitro IC₅₀ values for ANI in combination with either AS or CQ against Plasmodium isolates.

Combination	Strain	Combination quantities	FIC; AS or CQ	FIC; ANI	Sum FIC
AS/ANI	3D7	0.8 ng/ml AS + 2.6 μg/ml ANI	0.5	0.36	0.86
	W2	1.1 ng/ml AS + 3.3 μg/ml ANI	0.45	0.37	0.82
CQ/ANI	3D7	4.6 ng/ml CQ + 3.1 μg/ml ANI	0.42	0.43	0.85
	W2	22 ng/ml CQ + 3.7 μg/ml ANI	0.45	0.42	0.87

The drug combination concentrations were based on concentrations inhibiting 50% growth of parasite. With sum FIC values <1, these combinations are synergistic against the parasite.