Primary Cilia Blockage Promotes the Malignant Behaviors of Hepatocellular Carcinoma via Induction of Autophagy

Figure 2

Primary ciliogenesis blockage promotes the malignant behaviors in vitro. (a) SMMC-7721 and Huh7 cells were transfected with si-IFT88 or si-NC (negative control) for 72 h. The expression of IFT88 was determined by western blot analysis; the value under the band is the ratio of the blot and normalized to control. (b) Representative immunofluorescence images of cilia marker acetylated tubulin (×200). (c) Length of primary cilia. (d) SMMC-7721 and Huh7 cells were transfected with si-IFT88 or si-NC (negative control) for 48 h. Cell viability was then measured by CCK-8 assay for another 24 h, 48 h, and 72 h, respectively. (e, f) Migration and invasion ability were measured by transwell assay for another 24 h (×200). , .

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