Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification

Table 1

Sequences and binding temperatures for RT-qPCR (temp).

Gene	Sequence fwd (5′ ⟶ 3′)	Sequence rev (5′ ⟶ 3′)	Temp (°C)
GADPH	ATGACGAGGGCCTGGAGTGTG	CCTATGTGCTGGCCTTGGTGAG	60
VEGF	ATGACGAGGGCCTGGAGTGTG	CCTATGTGCTGGCCTTGGTGAG	60
TGF-β1	GCGTGCTAATGGTGGAAAC	CGGAGCTCTTGATGTGTTGAAGA	60
PEDF	AGTTACGAAGGCGAAGTCACCAAGTC	GCCCGGTGTTCCACCTGAGTC	50