Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats

Figure 4

Light photomicrographs stained by H&E. (a) CG showing the cords of hepatocytes radiating from the central vein (CV) and separated by slit-like sinusoidal spaces (red arrow) lined by endothelial cells (arrow head) (mic. mag.: 400×). (b, c) LFG showing the disorganized hepatic architecture. Many centrilobular hepatocytes have swollen vacuolated cytoplasm (green arrow) with obliterated blood sinusoids in between. Periportal hepatocytes with vacuolated cytoplasm and karyolitic nuclei (arrow head) are observed. Many hepatocytes have deeply stained eosinophilic cytoplasm and dark nuclei (black arrow). Proliferated bile ducts (b) and periportal mononuclear cellular infiltration (mn) are also seen. Few hepatocytes show increased nucleocytoplasmic ratio (red arrow) (mic. mag.: 400×). Inset in (b) shows rosette shaped aggregates (circle) (mic. mag.: 400×). Inset in (c) shows cells having vacuolated cytoplasm and karyolitic nuclei (arrows) with obliterated blood sinusoids in between () (mic. mag.: 400×). (d) MSCs + CCl₄G showing disorganized hepatic architecture with obliterated sinusoids. Many hepatocytes reveal swollen vacuolated cytoplasm and deeply stained nuclei (arrow) radiating from congested central vein (CV) (mic. mag.: 200×). (e, f) MSCsG showing restored architecture of the hepatic lobules. The hepatocytes are arranged in cords radiating from the central vein (CV) and separated by patent blood sinusoids. Centrilobular hepatocytes appear with eosinophilic granular cytoplasm and rounded vesicular nuclei (arrow) ((e): 200×; (f): 400×).

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