Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats

Figure 8

(a–d) Light photomicrographs of immunofluorescence-stained liver sections for the expression of TGFβ1. (a) CG showing the minimal expression of red fluorescent TGFβ1 (arrow) mainly in the cytoplasm of individual hepatocytes (H) and sinusoids (S). (b) LFG showing the strong expression of red fluorescent TGFβ1 in the perinuclear cytoplasm of many hepatocytes (arrow). (c) MSCs + CCl₄G showing the moderate expression of red fluorescent TGFβ1 in the perinuclear cytoplasm of hepatocytes (arrow). (d) MSCsG showing the minimal expression of red fluorescent TGFβ in the cytoplasm of few hepatocytes (arrow) (confocal microscopy, anti-TGFβ1, Hoechst nuclear stain, mic. mag.: 63×). (e) A morphometric study applying manual counting for the mean number of cell fluorescence for TGFβ1. Values represent mean ± SD. Different letters indicate significant statistical differences at . ^aSignificant compared with CG1. ^bSignificant compared with CG2. ^cSignificant compared with CG3. ^dSignificant compared with CG4. ^eSignificant compared with LFG. ^fSignificant compared with MSCs + CCl₄G. It reveals that CCl₄ treatment leads to significant increase in the total cell fluorescence of TGFβ1 which is reduced following BM-MSCs treatment.

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